The affinity-purified shelterin was then subjected to multiple rounds of SEC, using a Superose 6 Increase 10/300 column equilibrated with the SEC buffer (50 mM HEPES pH 8.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP) to further purify and polish the complex
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8 Belosic HalleZ.VlainicJ.DrmicD.StrinicD.LueticK.SucicM.et al (2017)
Live-cell imaging demonstrated that the rate at which newly nucleated microtubules emanated from the centrosome was decreased in cells adhered by signaling-defective integrins [17]
Because B12 is primarily found in animal products (meat, fish, dairy, eggs), people following vegetarian or vegan diets may be at higher risk of deficiency