Gunesch S, Hoffmann M, Kiermeier C, Fischer W, Pinto AFM, Maurice T, Maher P, Decker M (2020) 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
Post-induction, cells were harvested, sonicated, and the supernatant was subjected to Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva)
Bar graph showing knockdown efficiency of ZDHHC14 or ZDHHC23 at the mRNA level ( n = 3 independent experiments)
Nat Struct Biol
Resuspended resin just means that you want to invert the bottle of agarose resin a few times to make sure that all the beads are off the bottom of the bottle and in solution before you pipet some out of there
(2021) 34:e4309