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doi: 10.1016/j.cbpa.2017.07.006

Koyun et al., Biosensors and their principles, 117142 (2012) R

NAD + -consuming enzymes include NAD + glycohydrolases (NADases) such as CD38 and sterile alpha and TIR motif containing 1 (SARM1), deacylases (sirtuins), and ADP-ribosyl transferases (PARPs)

After blocking, the cells were rinsed for three times with TBST buffer (PBS supplemented with 0.5% Triton X-100 and 0.5% Tween-20) for 5 min per wash to eliminate excess blocking reagent