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The digest was filtered through a 70-m mesh and centrifuged using a 28.7% Nycodenz gradient (Nycomed PharmaAS, Oslo, Norway)

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The authors concluded that oral collagen supplementation is effective in reducing wrinkles and enhancing skin elasticity and hydration, which was likely due to the promotion of extracellular matrix biosynthesis [54]

Physical Exam Initial vital signs: BP 122/82, HR 68 BPM, Temp 98.6 F, RR 20, and SpO2 100% on room air