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After discarding the supernatant, the cell pellet was resuspended in Matrigel, and 50 L droplets were plated into each well of a 24-well plate

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CDNB, 2,4-dinitrochlorobenzene

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In pharmacological assessments of Long R3-IGF-1, human plasma analysis confirmed measurable bioavailability for over 24 hours post-administration, demonstrating sustained receptor engagement