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The column was washed with 25 mM Tris (pH 7.5), 200 mM NaCl and 10 mM imidazole, and eluted with 25 mM Tris (pH 7.5), 200 mM NaCl and 500 mM imidazole
This does not mean peptides are useless
For the MOTS-c 5-amino-1MQ stack protocol, researchers commonly administer MOTS-c subcutaneously three to five times per week, often timed around fasted training sessions or periods of caloric restriction to maximize AMPK activation
