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Antiviral Resistance In Cell Culture and Biochemical Assays SARS-CoV-2 Mpro residues potentially associated with nirmatrelvir resistance have been identified using a variety of methods, including SARS-CoV-2 resistance selection, testing of recombinant SARS-CoV-2 viruses with Mpro substitutions, and biochemical assays with recombinant SARS-CoV-2 Mpro containing amino acid substitutions

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Abdel-Wahab BA, Metwally ME, El-khawanki MM, Hashim AM