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Slides were placed in lysis solution for 1 hour, followed by 40 min denaturation in electrophoresis buffer (pH 13) and then electrophoresis for 30 min (25 V, 300 mA)

That slows down their ability to heal and to protect the roots of your teeth

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We agree that VTA neuron identification requires careful consideration, and we now provide new data and new Fig 1 to address the reviewers concerns and clarify our identification criteria