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doi:10.1074/jbc.M805586200, PMID: 18990698 Mieyal JJ, Starke DW, Gravina SA, Hocevar BA (1991) Thioltransferase in human red blood cells: kinetics and equilibrium

F.PanditJ.2000Identification, Characterization, and Crystal Structure of the Omega Class Glutathione Transferases.275322479824806 12

A denaturing agent, such as 8M urea, will also break the interaction by altering the configuration of the site where the antigen binds molecule of the antibody 4 Step : Affinity medium is re-equilibrated with binding buffer

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Reconstituted material should be stored only under controlled conditions appropriate for short-term analytical use

It's the linchpin of your entire experimental protocol